Jammy said
Please correct me if im wrong but i think the thing that Wolfe cant stand is keeping quiet about the mole, he tried to talk to H a couple of episodes previous and H asked him to trust him he will sort it. I am assuming its part of the same plot.
I believe you're right about that. The reason he said 'he can't take this anymore' is because - like you said - he had to keep quiet. Considering Ryan and Horatio knew there was a mole, and Ryan was in a sense involved because Erica was the one who told him about it, he was expressing his frustration toward Horatio who told him that he'd handle things.